Flow Cell Path Length - GE AKTA start Maintenance Manual

3 Maintenance operations
3.5 UV Monitor

3.5.3 Flow cell path length

3.5.3 Flow cell path length
Description
A Flow cell path length test is used to derive the actual path length of the UV flow cell.
The test should be performed when the UV Monitor or the UV flow cell has been replaced,
and when normalized UV absorption comparisons between different systems are
needed.
Parameter description
Parameter
Cell
Abs
C drft
C amb
Prerequisites
•
•
44
NOTICE
Do not change the C drft and C amb values. These parameters
must be set by a GE Service Engineer.
Buffer A: immerse Buffer A inlet into demineralized water.
Buffer B: freshly prepared 1.0% acetone solution (vol/vol), which is expected to give
an absorbance value of 340 mAU.
Description
Path length of the UV flow cell (mm).
The absorbance of the liquid in the UV flow cell (mAU).
UV LED drift compensation constant.
Ambient temperature compensation constant.
ÄKTA start Maintenance Manual 29060308 AD