Ettan Ipgphor 3 Instrument - GE Ettan IPGphor 3 Operating Instructions Manual

3 System description

3.2 Ettan IPGphor 3 instrument

3.2 Ettan IPGphor 3 instrument
Platform
The platform accommodates one manifold for the first dimension isoelectric focusing
(IEF) run. Up to 12 fixed-length strip holders can be placed on the platform for rehydration
and IEF. The embedded electrode areas provide the electrical connection between strip
holder or manifold electrodes and an integrated high-voltage DC power supply delivering
up to 10 kV. Platform temperature is controlled by Peltier thermoelectric modules.
Both electrode areas are gold-plated copper. Placing a manifold or a strip holder so that
it bridges both electrode areas completes the electrical circuit (once the safety lid is in
position).
Protective cover
When analyzing proteins labeled with light sensitive stains like CyDye™, a light protective
cover is used. The cover is applied by magnetic force but is easy to partially remove for
checking the isoelectric focusing procedure, especially the migration of bromo-phenol-
blue at start.
The voltage is cutoff when the safety lid is opened.
32 Ettan IPGphor 3 Operating Instructions 28-9635-51 AC