GE AKTA pilot 600 User Manual page 66

4 Operation
4.1 Sample application
4.1.2 Apply sample from a system inlet
Setting
Interrupt sample appli-
cation at UV
Fractionation settings
Priming the inlet tubing
manually
The sample inlet tubing can be primed manually if desired, using manual instructions
or the Process Picture.
Follow the steps below to prime one sample inlet tubing manually using the Process
Picture.
Step
1
66
Description
If this option is selected, sample application will be inter-
rupted when the UV signal reaches the specified level. This
feature is typically used in adsorption chromatography, to
detect sample in the flowthrough.
Settings specified here determine how flowthrough during
sample application will be collected. See
tionation, on page 71
Fractionation during sample application is generally rele-
vant only for adsorption chromatography.
Action
Set the following modules:
Inlet A to inlet used for sample (for example, A2).
•
Air trap to By-pass.
•
Mixer (if installed) to By-pass.
•
Column valve to Waste 2.
•
Result: The flow path is opened as shown.
Section 4.2 Frac-
for details of fractionation settings.
ÄKTA pilot 600 User Manual 29274559 AA