GE Typhoon FLA 7000 Getting Started page 91

Getting Started with Typhoon FLA 7000 28-9607-64 AD
Hluti Lýsing
3 Comment: enter an optional comment. The comment is embedded
in the file where the image is saved, and can be viewed with the
analyzing software.
4 Displays the laser and the filter used. In phosphorimaging mode,
the laser is automatically set to 650 nm, and the filter is autom-
atically set to IP.
Efnisyfirlit
To use the phosphorimaging mode, the 650 nm laser must be loaded,
and the IP filter must be set. If these conditions are not fulfilled, the
Reader Settings window for the phosphorimaging mode cannot be
accessed.
5 PMT: set the voltage of the photo-multiplier tube. The higher the
value, the higher the sensitivity.
6 Set the scanning area.
1 Drag the red square to the desired position of the scanning
area.
2 Drag the sides of the red square as needed to adjust the size
of the scanning area.
7 Pixel Size: set the pixel size.
Choose a small pixel size for high quality images. Note that a small
pixel size setting increases the reading time and the size of the
image file.
8 Latitude: Specify the dynamic range.
L5 provides a larger detectable range than L4.
•
L4 provides a finer density gradation, if the signals of the sample
•
areas are in the correct range.
9 Mode: Specify the mode for the reading area borders.
Select Grid to limit the borders to the grid lines.
•
Select Free for an arbitrary area.
•
Select All to use the entire Fluor stage as the reading area.
•
10 Save Condition... click this button to save the current reading
conditions in a file, if desired. For details, refer to the User Manual.
7 Reading phosphorimaging samples
7.1 Set the reading conditions
91