Preparations Before Start - GE AKTAprime plus Operating Instructions Manual

4 Operation
4.2 Starting the instrument
4 The self-test takes about 30–40 seconds. When the test is completed, the display
Note:
4.3

Preparations before start

Buffer preparation
Prepare buffers according to ÄKTAprime plus cue cards.
Sample preparation
1 Adjust the sample composition to the binding buffer by:
2 Filter the sample through a 0.45 µm filter.
Purification setup
Removing storage solution from the flow path
At delivery and during storage, the flow path is filled with 20% ethanol. This should be
removed before continuing the setup.
Note:
To flush out the ethanol using deionized water:
1 Put the inlet tubing A1–A8 that is used and B in deionized water.
2 Put all waste capillaries, W1–W3, in waste.
3
4 Select Application Template and press OK.
5 Select System Wash Method and press OK.
34
shows the Templates menu.
The system can be used for most applications after 15 min of lamp warm-up
but the full specifications are not obtained until after 1 hour.
•
diluting the sample in binding buffer, or
•
buffer exchange using HiTrap™ Desalting or HiPrep™ 26/10 Desalting column.
Do not use buffer with high salt concentration to flush out the ethanol. It might
cause too high backpressure.
Note:
At delivery, only A1 and B are installed.
Select Templates in the main menu using the and buttons and press OK.
ÄKTAprime plus Operating Instructions 28-9597-89 AB