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d. TRANSFER the TEM grid into the recess
at the end of the holder using the
tweezers.
e. Use the pin tool to gently LOWER the
clamp straight down to hold the grid
securely. RETURN the tool to the base of
the holder stand. To make scanning the
grid easier, you may wish to orient one
set of grid bars parallel to the long axis of
the specimen holder.
f. RETRACT the holder slightly and turn it
upside down. TAP the back end several
times, then turn the holder upright and check that the grid has not moved (movement
suggests the grid is not properly secured).
g. Use the microscope to inspect the holder O-ring for debris. Gently remove any debris
using a sheet of Kimwipe.
C. Inserting the Sample Holder into the CompuStage
a. Carefully ORIENT the Airlock (Sample Holder) Pin on the sample holder with the 5
o'clock position on the CompuStage and gently INSERT the holder into the Airlock
Entryway until it stops. Be careful NOT to scrape the tip on the inner mechanism of
the goniometer. You should feel some resistance as the sample holder O-ring seats
in the Airlock Chamber.
b. Before inserting the holder into the column, the holder needs to be pre-pumped until
the indicator is off.
c. The data monitor screen will automatically open to the Holder Selection Page. PRESS
the appropriate key and then PRESS the Ready button (below the data monitor
screen).
d. The Airlock will begin pumping and the Red LED on the CompuStage will illuminate.
Do NOT move the Sample Holder when the Red LED is illuminated.
2017.07.07
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