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5. Focusing the Image and Diffraction Pattern
a. Focusing the image with the image wobbler
i. Set the Focus Step size (inner Step Size knob) to 5 and PRESS the D button to place
the microscope in Diffraction mode.
ii. ADJUST the Camera Length to 620 mm (NOT 6.2 m) using the Mag knob.
iii. ADJUST the Beam Intensity.
iv. Center the Objective Aperture and ACTIVATE the Focus Wobbler.
v. USING the Multifunction X/Y, ENUSRE the two spots are centered in the Objective
Aperture area.
vi. SELECT D, FOCUS (minimum blur), DEACTIVATE the Wobbler
6. Camera Control and Imaging
a. CREATING a new Background Image. Note: The background image compensates for
phosphor inhomogeneity and optical imperfection.
i. VIEW the electron beam on the TEM viewing screen.
ii. CENTRE the beam.
iii. REMOVE specimen from column or locate a hole on TEM grid
iv. SET the magnification to roughly the magnification you will take the images.
v. SPREAD the beam with C2 control clockwise so that the current is about 1.0
second on the exposure meter.
vi. LIFT the viewing screen and cover the viewing glass window with the black rubber
mat.
vii. On the AMT software, SELECT the menu item Corrections --> Acquire Background.
viii. ADJUST the intensity knob (C2) so the histogram on the AMT software is
approximately centred in the box. When this is correct, PRESS the orange
command button.
ix. It will take ~60 seconds for the background correction to be completed. WAIT until
the Click Live Imaging button is re-enabled.
b. On the AMTv600 program, PRESS Live Image.
2017.07.07
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