Sterilization Objects - Panasonic MLS-3751 Operating Instructions Manual

High-pressure steam sterilizer
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Sterilization Objects

・Do not sterilize objects in a steam impermeable
container or bag.
Failure to observe this warning may result in accident or
incomplete sterilization.
・Do not seal the object container tightly.
Use a porous cap or loosen the cover sufficiently.
Failure to observe this warning may result in accident or incomplete sterilization.
・Do not block the holes or temperature sensor in the
chamber with objects.
Failure to observe this warning may destabilize control of the
product or result in incomplete sterilization.
Sterilization and melting of liquids such
as culture mediums, etc.
Capacity of culture mediums and containers
Pour less liquid in the container than the specified volume.
Excessive liquid will spill when heated.
Sterilization of large volume culture mediums
Set the timer to a longer time than usual.
There is a time lag (time difference) from when the temperature in
the chamber reaches the sterilization temperature until the
temperature of the whole culture medium reaches the sterilization
temperature.
Sterilization Setting Time = Sterilization Time + Time Lag
Liquid Volume
Agar media in 500 ml flask x 1~21, or
Agar media in 1000 ml flask x 1~12
Recommendation
An "Object temperature sensor" that detects the temperature
of objects to be sterilized and controls sterilization time is available
as an option. Even after this sensor has been installed, control can
be switched over to the product ' s own sensor by pressing the
object temperature ON/OFF button. Use this optional sensor to
ensure sterilization is more reliable. For details, contact your
dealer.
Sterilization with the use of plastic containers
Prolong the sterilization setting time even more. Otherwise, poor
heat conduction of the container may cause incomplete
sterilization.
Melting of solidified general agar mediums
Refer to the below table to set the melting time (per flask).
Capacity
Melting Temperature
2 L
1 L
Test of Durham tube
Air remaining in the Durham tube can be purged by setting the
exhaust temperature and exhaust rate to appropriate levels.
WARNING
Time Lag
MLS-3751
8 min.
Melting Time
100
°
C
Temperature
121 °C
100 °C
MLS-3781
11 min.
60 min.
45 min.
Porous caps
Sterilization
setting time
Sterilization
Time lag
time
Temperature inside
the chamber
Culture medium
temperature
Time
Plastic
container
Durham tube
13

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