HP D300 Operating Manual page 44

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To start from a new Fluids List to design a new plate configuration, select Clear all data
and start from scratch (no fluids, empty well plate).
Figure 5-17 Plate Layout Wizard: Initialization dialog box
If you selected the first option, the next two questions involving assay volume and the
b.
DMSO limit will be unavailable because the previous values are used. If you selected the
second or third option, type the assay volume and DMSO limit for the new plate design into
the boxes and press the ENTER key on the keyboard.
The next question involving the number of fluids you are using also changes depending on
c.
which option was selected above. Read the question carefully, type an answer into the box,
and press the ENTER key.
Click in the first column of the table to edit the names of the additional fluids, if desired, and
d.
then type the source plate/vial, source well, and concentration information into the following
columns.
Select the NoDispense check box if the fluid should be a no dispense fluid (fluids are
e.
which are non-HP dispensed fluids). There needs to be at least one fluid with NoDispense
checked in order to reserve rows or columns for controls which are not dispensed by the
HP dispenser.
To apply a concentration other than the default, type the appropriate value in the box at the
f.
bottom of the Initialization dialog box and click the Use this concentration for all fluids
button to apply the new value to all of the new fluids.
Click Continue to move to the next step. The Plate Layout Wizard: Titrations and
g.
Positioning dialog box appears. See
IMPORTANT: Once titrations and positioning are initiated, changes cannot be made to the
fluids table because changes could disrupt the design. To make changes to the fluids table,
click Cancel, then make changes to the fluids table before launching the Plate Layout
Wizard again.
Answer these
questions unless
you chose to leave
all existing data
Type fluid
information
in this area
if desired
Figure 5-18.
HP D300 Digital Dispenser
Select one of these
three options
Select at least one
NoDispense to
allow placement of
controls
Change concentration
here if desired
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