Create Titration2 Layouts: Two Fluids Per Well - HP D300 Operating Manual

Figure 5-30

5-10-3 Create titration2 layouts: two fluids per well

A titration2 layout creates a series of wells for each combination of fluid concentrations. For
example, if each fluid is designed as a twelve-point singlet, Titration2 will create a 12 × 12 matrix of
144 wells of fluid combinations unless the ―Remove points in the high side triangle‖ option is
selected as described below.
1. To set up a titration2 layout with two fluids per well in a systematic design, follow steps 1 and 2
in 5-9-1 above,
2. Click Titration2 in the left pane of the Plate Layout Wizard: Titrations and Positioning dialog
box. This opens the Titration2: <none> in the left pane of the Plate Layout Wizard: Titrations
and Positioning dialog box. The Titration2 pane is opened as shown in
3. Select two fluids you would like to use for this dual fluid titration by selecting the Use check
boxes.
Titration1 pane showing two entries in the Conc. (mM) column
HP D300 Digital Dispenser
Figure 5-31.
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