GE AKTA avant User Manual page 176

5 Operation
5.4 Prime inlets and purge pump heads
5.4.2 Prime sample inlets and purge Sample Pump
Step
5
6
7
8
176
Action
In the Process Picture:
Click the Sample inlet icon, then click Buffer.
•
Click the Sample pump icon: Set the Sample flow to 1.0 ml/min for ÄKTA
•
avant 25 or 5.0 ml/min for ÄKTA avant 150.
Click Set flow rate.
•
Result: The sample pump flow starts.
Connect a 25 to 30 ml syringe to the left purge valve of the sample pump.
Make sure that the syringe fits tightly into the purge connector.
Open the purge valve by turning it counter-clockwise about three-quarters
of a turn. Draw 5 to 10 ml of liquid slowly into the syringe with a rate of about
1 ml/s.
Close the purge valve by turning it clockwise. Disconnect the syringe and
discard its contents.
ÄKTA avant User Manual 29035184 AE