3.11 Uv Monitor - GE AKTA avant User Manual

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Table of Contents

3 Standard instrument modules

3.11 UV monitor

Introduction

This section describes the design and function of UV monitor U9-M. The module includes

a monitor unit and a detector with a UV flow cell.

Function of the UV monitor

UV monitor U9-M measures the UV absorbance at a wavelength range of 190 to 700

The UV monitor is automatically calibrated every time the instrument is switched on.

A flip-mode enables measuring of UV/Vis absorbance at three wavelengths simultane-

ously during a run. The second and third wavelength can be turned off or on in method

phase properties, by manual instructions or in system settings.

Location and illustration of UV

The following illustration shows the location of the UV monitor and also an enlargement

of the monitor unit and detector. In both ÄKTA avant 25 and ÄKTA avant 150 the monitor

unit is labelled U9-M and the detector U9-D.

The resolution is decreased when more than one wavelength is used simulta-

neously due to lower sampling frequency per wavelength. Do not use more

wavelengths than necessary.

ÄKTA avant User Manual 29035184 AE