GE AKTA avant User Manual page 453

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Predefined purification

Reversed Phase Chro-

matography (RPC)

Predefined maintenance

A number of predefined methods for preparation and cleaning are available. These

maintenance methods are used to prepare the system, clean the system, and to fill the

system with storage solution.

The following table describes the available predefined maintenance methods.

ÄKTA avant User Manual 29035184 AE

After equilibration and sample application,

hydrophobic proteins adsorb to the column

ligand. After a wash to remove unbound

sample, elution is performed by generating

a gradient of a non-polar, organic solvent

such as acetonitrile. Finally, the column is

washed and re-equilibrated.

Fraction collector. Do not fractionate flammable liquids in the

built-in fraction collector. When running RPC methods, collect

fractions through the outlet valve or the optional external Fraction

collector F9-R.

9 Reference information

9.6 Predefined methods and phases

Included phases

Troubleshooting