Prime Q Inlets - GE AKTA avant User Manual

5 Operation
5.4 Prime inlets and purge pump heads

5.4.3 Prime Q inlets

5.4.3 Prime Q inlets
Overview
The procedure consists of the following stages:
Stage
1
2
3
4
5
Prime the Q inlets
Follow the instructions to prime the Q inlets.
Step
1
2
178
Description
Prime all Q inlet tubing.
Validate priming of Q inlet tubing.
Purge Quaternary Valve and the system pumps if pressure signal indicates
air bubbles.
Validate purge of Quarternary Valve and system pumps.
End the run.
Action
Make sure that the pieces of inlet tubing marked A1, B1 and Q1-Q4 are im-
mersed in the correct buffers. The A1 and B1 positions are used for pump
synchronization and these lines should already be primed.
In the Manual instructions dialog box:
Select Pumps and pressures:Quaternary start concentrations.
•
Set Start concentration Q1 to 100%. Make sure that the other start
•
concentrations are set to 0%.
Select Pumps and pressures:System flow and set Flow rate to 0.01
•
ml/min.
ÄKTA avant User Manual 29035184 AE