Photometry Assay Concentration Calculation; Calculation Of K Factor; Calculation Of S1Abs - Hitachi 902 Service Manual

9.3

Photometry Assay Concentration Calculation

This calculation is exemplified below on ALB.
Assume that standard solution 1 (reagent blank) and standard solution 2 have concentrations of
0.0 g/dL and 5.3 g/dL, respectively.
Calculation of S1ABS (absorbance of standard solution 1 × 10
448 + 445
S1ABS =
2
"447" is indicated on the monitor menu 2 screen.

Calculation of K factor

−
C C
2 1
K =
−
A B
2
C : Concentration of standard solution 2
2
C : Concentration of standard solution 1
1
Printed absorbance of standard solution 2 × 10
A :
2
Printed absorbance of standard solution 1 × 10
B :
In the calculation of K value, each concentration value is rewritten into the integral part and
exponential part, and calculation is carried out while ignoring the fractional digits (and the
exponential part). The number of fractional digits is counted with reference to the concentration
value of standard solution 1. Therefore, 0.0 is rewritten into 0 × 10
values 0 and 53 are used for calculation.
The value 202 obtained by calculation is indicated on the monitor menu 2 screen.
K =

+

3050 3097




2
Rounding off
= 446.5
−
53 0

+
  
448 445
 −  ×
 
10
  

2
447
-4
-4
53
=
( )
− ×
3074 447 10
−
4
9 - 9
4
)
, and 5.3 into 53 × 10
-1
=
202
−
4
-1
, and