Hitachi ChromasterUltra Rs 6270 Instruction Manual page 49

Figure 2-8a All Volume Injection Method
(Sample loops: 40, 100µL)
(b) All Volume Injection Method (Air = 0 µL)
When applying all volume injection method (Air = 0 µL),
the loop capacity more than the injection volume is
required.
Select loop capacity within the area indicated in the
following figure.
The sample introduction volume is selected within the
shadow area of the following figure. (For example, 40 µL
loop is required when measuring 10 µL sample. This
means that the sample volume requires quintuple loop
capacity) (Sample loops: 40, 100, 1000 µL).
(c) Full Loop Injection Method
When applying full loop injection method, the sample
introduction volume more than quintuple (up to decuple)
loop capacity (injection volume) is required (amount of
sample necessity). (For example, 1 µL loop is required
5 to 10 µL sample when measuring.)
(2) Wash fluid selection and use examples
The ChromasterUltra Rs 6270 Autosampler uses two kinds of
wash fluid. Refer to the following table.
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Figure 2-8b All Volume Injection Method
(Sample loops: 1000µL)
2.2.2