Hitachi ChromasterUltra Rs 6270 Instruction Manual page 87

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(g)
vd: Dead volume (the instrument inside parameter)
vi: Injection volume (the sample net volume to the flow path)
vw: Waste volume (the air volume of the lead and rear part of a sample)
va: Air volume (the air volume which sample is sandwiched.)
Figure 2-41 Discharging the Sample into the Sample Loop
Loading a sample
The syringe discharges the one-half of the air volume
(va/2) + one-half of the waste volume (vw/2) + injection
volume (vi) + dead volume (vd) (inside parameter) of
sample into the sample loop.
2 - 47
2.2.3

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