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4.4 Selection of Mobile Phase and Cautions on Handling
Table 4-1 Reagents for Mobile Phase that may be Restricted
Use is Possible
Phosphoric acid
Sodium phosphate
Use is Possible If
50% or Lower
Acetic acid
Ammonium citrate
Ammonium nitrate
Citric acid
Butyric acid
Sodium nitrate
Do not use reagents that corrode PEEK resin or SUS316 though
they are not shown in the table above.
(5)
Concerning the Washing the Flow cell
When normally unsuitable reagents to PEEK resin or
stainless steel that are indispensable for component
separation or reaction of a constituent have been used, be
sure to wash out all the flow paths with the suitable solvent
(e.g. methanol or acetonitrile when an organic solvent is
used as the mobile phase, or distilled water when an
aqueous solution is used as the mobile phase) after the
completion of analysis. Failure to carry out washing may
cause to clog or damage the flow cell.
(6)
Concerning the Using Buffer Solutions
After using a buffer solution or the like, make sure to wash
out the flow cell with distilled water. Failure to carry out
washing may allow residual salt to precipitate and clog or
damage the flow cell.
Use is Possible If 10%
or Lower
Disodium phosphate
Ammonium formate
Ammonium perchlorate
Ammonium phosphate
Boric acid
Formic acid (up to pH3)
Hydrochloric acid (up to
pH3)
Potassium nitrate
Sodium
hydrogencarbonate
Sodium carbonate
4 - 4
Reagents Unsuitable
Tetrahydrofuran
Dichloromethane
Chloroform
Acetone
Dimethylsulfoxide
(DMSO)
Ammonium chloride
Potassium chloride
Sodium chloride
Tolufluoroacetic acid
Note 1:
Max. concentration of
above reagents should
be 5-6%.
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