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6.1 Troubleshooting
Symptom
5. Noise is too
large.
6. Drift is
excessive.
Cause
(1) Flow cell is
contaminated.
(2) Solvent is not
degassed
adequately.
(3) Bubbles are
formed in flow cell
because its back
pressure is
inadequate.
(4) Impurities are
contained in
solvent.
(5) Trouble has
occurred on
pump.
(6) D
Lamp has
2
reached the end
of its lifetime.
(1) Warm-up is
insufficient.
(2) Liquid is leaking
through a joint.
(3) Impurities are
eluted from
column.
(4) Ambient
temperature
changes
significantly.
(5) Use of low-purity
solvent
Judgment/Check
Spike noise appears.
Spike noise appears.
Check the solvent.
Check for regularity in
occurrence.
Check for noise at pump
stop.
Check lamp energy,
referring to 5.2.
-
Check fastening of the
joint.
Stop liquid delivery from
pump and check that the
baseline stabilizes.
-
Check the solvent.
6 - 2
Remedy
Clean the flow cell,
referring to 5.4.1.
Degas the solvent
adequately.
When flow rate is low
(below 0.2 mL/min) or
the viscosity of
mobile phase is low,
a tube for back
pressure (0.25 mm
I.D. 1 m Teflon tube
or the like) should be
added. For details,
contact the nearest
maintenance service
company authorized
by Hitachi High-
Technologies.
Purify or renew the
solvent.
Make sure the pump
is normal.
-
Replace the lamp
with a new one,
referring to 5.7.1.
Wait until signals
stabilize (at least 1
hour including the
time for lamp mode
change).
Retighten the joint.
Wait until impurities
have been eluted
completely or replace
the column with a
new one.
Minimize ambient
temperature change.
Purify or renew the
solvent.
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