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5.4 Maintenance/Check Procedure
Inflammation or injury due to toxic, corrosive or
When using a toxic, corrosive or stimulative solvent, be
careful not to incur inflammation or injury. For details of the
properties of each solvent and how to handle it, refer to
Safety Data Sheet (SDS). Be sure to handle each solvent
properly.
(a)
Wear proper protective means (e.g., safety goggles)
so that a solvent will not come into direct contact with
the skin, eye, etc.
(b)
Ventilate the laboratory room adequately to prevent
accidental inhalation of harmful solvent vapor.
If the lamp energy does not recover after replacing D
there may be contamination inside the capillary or the light inlet
or outlet of the flow cell unit. In the case, cleaning is required.
Items to be prepared for flow cell cleaning:
Pump
Detergent
Lens cleaning paper or cotton swab
Methanol
Check and clean the flow cell in the following procedure.
(1)
Deliver detergent into the flow cell with pump and wash the
contamination out of it.
Detergent to be used varies with the mobile phase which
has been used.
When the mobile phase used is aqueous, deliver distilled
water with the pump at flow rate 0.5 mL/min or lower and
pressure 6.0 MPa or lower at first for cleaning, and deliver
ethanol, etc. when the mobile phase used is an organic
solvent.
NOTICE: If the flow cell is pressurized excessively, it may be
broken. Be sure to set the pressure limiter to max.
6.0 MPa before pumping liquid into the flow cell for
washing.
5 - 8
WARNING
stimulative solvent
lamp,
2
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