Storage - GE AxiChrom 50 Operating Instructions Manual

5 Operation

5.9 Storage

5.9 Storage
Residual chloride ions can be corrosive to stainless steel over time. Therefore it is critical
to rinse away any NaCl buffer that may have come into contact with any part of the
column during operation.
Storage of a packed column up
to 24 hours
The column may be stored with a packed bed and packing liquid for a recommended
period of no more than 24 h. Storage beyond this time may lead to microbial growth. If
within this 24 h period the pump or system to which the column is connected is needed
for other purposes during the storage period of the column, it is appropriate to fit the
mechanical locking equipment (see Mechanical locking, on page 39) onto the column.
Plug the mobile phase inlets/outlets and the hydraulic chamber inlet. Ensure that the
storage conditions of the column do not cause an increased pressure in the column.
Storage of a packed column for
longer than 24 hours
For storage periods longer than 24 h, it is recommended that the process chamber
containing the medium is filled with an appropriate antimicrobial agent, for example
20% (v/v) ethanol (check the instructions of the specific medium). Use mechanical locking
equipment to keep the axial compression (see Mechanical locking, on page 39).
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NOTICE
By tightening the locking nuts on the mechanical locking equipment
it is possible to work the adapter down and to compress the bed
more than intended during the packing of the column.
NOTICE
Low pH solutions containing chlorides can cause corrosion in
stainless steel. Rinse thoroughly with clean water after each use
with such solutions. Inspect the column regularly for signs of cor-
rosion, which will cause column damage if untreated.
AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG