GE AxiChrom 50 Operating Instructions Manual page 98

5 Operation
5.3 Manual packing of the column
Manual packing with a separate
packing pump
It is possible to pack AxiChrom 50, 70 and 100 with a separate packing pump.
1 Prepare the media slurry (see Preparing the slurry, on page 63). Remember to com-
2 Prime the column (see Priming the column, on page 73) and close the bottom mobile
3 Pour the slurry into the column (see Pouring slurry into the column, on page 74).
4 Fit the adapter and top plate onto the column (see Fitting the top plate and adapter,
98
CAUTION
Ensure that the pivot stand locking pin is secured before starting
the process so that the column cannot turn over by accident.
pensate for the compression when calculating the slurry volume needed to achieve
the target bed height. The target bed height is calculated from the consolidated bed
height and the Packing Factor.
Packed bed height =
phase inlet/outlet.
on page 41).
NOTICE
Ensure that the full flow path is unrestricted when pumping
liquid into the column.
NOTICE
Insert the adapter into the mouth of the column tube at an
angle to ensure that no air is trapped in the column.
NOTICE
Ensure that the top plate is completely closed by confirming
that the bayonet tool in the top plate is aligned with the ma-
chined mark on the top bayonet.
consolidated bed height
packing factor
AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG