Intelligent Packing Of The Column With Äkta Avant 150 - GE AxiChrom 50 Operating Instructions Manual

5.2 Intelligent packing of the column with ÄKTA avant 150
Intelligent Packing allows for convenient and precise control of the column packing using
AxiChrom column together with ÄKTA system, UNICORN software, and verified packing
methods. Several BioProcess™ media are available from GE Healthcare that have been
verified for use with AxiChrom column and Intelligent Packing. These are found in a list
of media in the Intelligent packing phase.
AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG
5.2 Intelligent packing of the column with ÄKTA avant 150
NOTICE
Ensure that the air trap is filled with mobile phase liquid. This is
because the methods created using the Intelligent Packing wizard
are constructed so that the airtrap is in line with the column when
equilibration and testing operations are being performed. These
require that flow occurs into the process chamber via the mobile
phase inlet/outlet. By contrast, during packing, the method is con-
structed so that the air trap is bypassed as hydraulic liquid is
pumped into the hydraulic chamber of the column.
5 Operation
79