GE AxiChrom 50 Operating Instructions Manual page 150

Columns
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7 Troubleshooting
7.2 Adapter
Problem
Wrong bed height
obtained.
150
Comment
The column was not correctly
leveled
Wrong packing buffer
Wrong amount of medium
added to the column.
Wrong Packing Factor ob-
tained.
AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG
Action
Level the column using the adjustable
feet and check with a spirit level.
Check selections made in the wizard,
particularly for bed height, bed support,
flow rate and Packing Factor.
Check the slurry concentration.
Ensure that the correct scale on the
column has been used. There is one
scale for stainless steel bed supports
and one for plastic bed supports.
Change to the packing buffer recom-
mended in the UNICORN Intelligent
Packing wizard. Repack the column.
Ensure that the correct slurry concen-
tration is entered in the Intelligent
Packing wizard and be careful to add
the right volume of slurry.
Check that defaults values were used
during Intelligent Packing. Performance
of media available in Intelligent Packing
wizards has been verified by GE in the
range default Packing Factor ±0.02.
Therefore it is known that a small devi-
ation in Packing Factor does not result
in bad performance.
Check that the container with hydraulic
liquid is not empty.
Replace scraper sealing if damaged.
Check that the pump used for packing
delivers the correct volume per time.
Unpack column and perform column
leakage test (see Section 6.4 Performing
a column leakage test, on page 143).

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