GE AxiChrom 50 Operating Instructions Manual page 76

5 Operation
5.1 Intelligent Packing of the column with ÄKTAexplorer and ÄKTApilot
76
b Follow the instructions on the screen to purge air from the hydraulic chamber.
The first instruction is to open the hydraulic chamber vent valve and press on
Continue. To do this, first loosen the plug to the vent valve and attach a syringe
without plunger. Then click on Continue.
As the air purging step proceeds the syringe will fill up with liquid from the hy-
draulic chamber. Click on Continue when no air bubbles are seen. This stops the
flow of liquid. Follow the instruction to seal the vent valve and remove the syringe.
c
Click on Continue again to start the consolidation.
d The bed consolidates as the adapter moves down in the column. The time taken
for the adapter to reach the surface of the consolidated bed is estimated during
method generation in the method wizard (Creating an Intelligent Packing method
using BioProcess media, on page 65) and can be viewed in the method notes.
Before the consolidated bed height is reached a warning dialog is displayed.
NOTICE
Make sure to remove the syringe otherwise it may become
hooked under the adapter rod top and hinder the packing,
especially if packing a low bed height.
NOTICE
Ensure that the column is free from air before the process
run is started.
NOTICE
Do not seal the hydraulic chamber vent valve too early, i.e.
wait for the instruction to close the vent valve. Otherwise
the adapter will be pushed down in the column without
UNICORN recognizing that the actual consolidation step
with timed countdown has started, and will require that the
bed is repacked.
AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG