GE AxiChrom 50 Operating Instructions Manual page 91

Step
8
AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG
5.2 Intelligent packing of the column with ÄKTA avant 150
5.2.2 Creating an Intelligent Packing method using ÄKTA avant 150
Action
In the Phase Properties pane of the Column Performance Test phase, make
sure that the settings are correct. After the equilibration two Column
Performance tests are run, one downflow and one upflow. Ensure that the
Upflow check box is checked in one of the Column Performance Test phases.
The test will then be run upflow. Ensure that the Upflow check box is
unchecked in the other Column Performance Test phase and this test will
be run downflow.
It is not necessary to run the tests, but is recommended to ensure the quality
of the packed bed. A solution of either acetone or NaCl can be used to give
a good indication of the column packing quality. The eluate is monitored by
measuring the UV absorption at 280 nm or the conductivity and the curve
can be evaluated in Evaluation. See Performance eveluation of the column
in [[ManualNameComplete]] for more information.
The illustration below shows the Method Outline pane to the left and the
Phase Properties pane of the Column Performance Test phase to the right.
Note:
To check if the test is run upflow or downflow, see the Upflow check box. If
this is checked, the test is run upflow.
At both tests the flow rate is set to 30 cm/h. It is important to use a low flow
and to use the same flow rate if tests are repeated to get comparable results.
5 Operation
91