GE AxiChrom 50 Operating Instructions Manual page 92

5 Operation
5.2 Intelligent packing of the column with ÄKTA avant 150
5.2.2 Creating an Intelligent Packing method using ÄKTA avant 150
Step
9
10
Creating an Intelligent Packing
method using custom media
Intelligent Packing methods may also be used for custom media. UNICORN facilitates
this by providing an extended range of variables that may be modified to optimize the
method. For example, it is possible to:
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•
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In the Phase Properties pane of the Intelligent Packing phase, make sure that the
Custom packing settings radio button is clicked.
92
Action
Click the Save the method icon.
Result: The Save As dialog opens.
In the Save As dialog:
Select a target folder to enable the Save button.
•
Type a Name for the method.
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Select a System from the list.
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Click the Save button.
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Result: The created method is saved in the selected folder.
Pack according to a desired Packing Factor or bed height. If selecting the latter option,
the user stops the compression when the final bed height has been reached.
Select flow conditioning with associated variables
Specify desired adapter velocity during packing within defined range
AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG