GE AxiChrom 50 Operating Instructions Manual page 47

3 Connect one end of a tubing to the bottom mobile phase in the bottom plate. Connect
4 Pump an appropriate liquid into the process chamber, at a low flow rate to begin
5 If the adapter is to be moved to its most upper position in the column when approach-
6 Ensure that the hydraulic chamber outlet and/or the hydraulic chamber vent valve
7 Ensure that the column is depressurized.
8 Insert a bayonet tool into each of the holes on the top plate [803] and top bayonet
9 Hold the tool still that is in the top bayonet and move the tool in the top plate coun-
10 Dampen the adapter rod [802] using a cloth wetted with 20% (v/v) ethanol and
AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG
the other end of the tubing to a system or pump.
with, while at the same time checking that the pressure does not rise above the
pressure limits of the column or medium. Increase the flow rate as appropriate while
maintaining a low column pressure. To avoid damage to the adapter scraper seal,
do not run the adapter faster than 120 cm/h.
If the adapter is not to be moved to its upper position, stop the pump when the
adapter has reached the desired height.
ing the top position, reduce the flow rate and continue the adapter movement until
the lower surface of the adapter is 2 cm below the lower surface of the top bayonet
and then stop the pump. This is to facilitate safe removal of the liquid above the
adapter.
are opened.
[804].
terclockwise to unscrew the bayonet fitting.
CAUTION
Make sure that the adapter is stable in the mouth of the column
or hold the adapter in an upright position.
carefully slide the top plate approximately 15 cm up the adapter rod while keeping
the adapter in place.
CAUTION
Beware when handling the sharp edges of the bayonet threads.
NOTICE
Never force the adapter out from the column manually. Only
use hydraulic pressure as described in this Operating Instruc-
tions to move the adapter in the column tube.
4 Getting started
4.6 Routine procedures
47