GE AxiChrom 50 Operating Instructions Manual page 97

14 Wash the system by running mobile phase at e.g. 150 ml/min for 3 minutes with the
15 Run the column upflow for a few minutes to remove residual air possibly trapped in
16 It is recommended to replace the tubing with a stop plug [101] at the check valve
AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG
column in Bypass mode.
•
In the System Control module, select Menu:Manual.
•
In the dialog, select Flowpath:InletA and select a wash inlet
•
Select Flowpath:Outlet and select an outlet.
•
Select Pump:Flow and enter the flow rate 150 ml/min.
•
Stop the pump after 3 minutes.
Note:
The instruction is for ÄKTApilot. The commands differ slightly for ÄKTAex-
plorer.
the adapter. The column is now packed. Before using it, evaluation of the packing is
recommended (see Section 5.4 Performance evaluation of the column, on page 100).
[116].
5 Operation
5.3 Manual packing of the column
97