GE AxiChrom 50 Operating Instructions Manual page 28

3 Specifications and characteristics
3.5 Chemical resistance
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7
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Chemical resistance to sodium
chloride and other chloride
containing liquids
Depending on configuration, AxiChrom columns are equipped with wetted components
of stainless steel and must therefore be appropriately maintained when exposed to
chloride ions during chromatographic processes. If a process incorporates the use of
chloride ion solutions it is essential that the column is thoroughly rinsed to remove any
residual chloride ions. It is recommended that a water rinse of at least five column vol-
umes is used. Water is the preferred rinse solution due to the solubility properties of
chloride ions in water versus other possible rinsing solutions like ethanol solutions. Both
the outside and the inside of the column should be thoroughly rinsed with purified water.
Residual chloride ions can be corrosive to stainless steel over time. Therefore it is recom-
mended to remove any buffer containing chloride ions that comes into contact with the
top bayonet during pouring of slurry into the column by rinsing with water. Also rinse
the upper surface of the adapter after placing it into the slurry. Columns should be stored
with solutions free from chloride ions.
It is also recommended to empty the hydraulic chamber before storage of a packed
column. Use a mechanical locking equipment to keep the axial compression (see Me-
chanical locking, on page 39).
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Not for use with columns containing wetted components of stainless steel.
pH below 4 for stainless steel is not recommended.
For columns containing wetted stainless steel components max. 1.0 M NaCl is recommended.
NOTICE
Make sure to rinse tubing and system after using Sodium hydroxide
to avoid damage of the tubing.
NOTICE
Low pH solutions containing chlorides can cause corrosion in
stainless steel. Rinse thoroughly with clean water after each use
with such solutions. Inspect the column regularly for signs of cor-
rosive attacks, which will cause column damage if untreated.
AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG