Wavelength Accuracy Check And Calibration - Hitachi PRIMAIDE 1440 Instruction Manual

4.2.4 Wavelength accuracy check and calibration

S
(baseline
method)
Baseline
Max.
wavelength
Fig. 4-5 Drift Measurement
NOTE: Carry out the drift measurement after the xenon
lamp has stabilized (at least one hour after the
lamp is turned on).
(1) Check of Wavelength Accuracy
(a) Principle of measurement
Wavelength accuracy of the emission side
monochromator is checked by using the bright line at
254 nm of the builtin Hg lamp.
As for the wavelength accuracy of the excitation side
monochromator, the Em side is fixed at the wavelength
position where the abovementioned bright line was
detected, then the Ex wavelength where a Rayleigh
scattering peak of 254 nm is produced is measured.
Raman spectrum of
water
S (tangent
method)
A
450 nm
Emission
wavelengths
Conditions:
Excitation(Ex)
wavelength ..................... 350 nm
Response ....................... 2 seconds
Flow cell .......................... 12 L
Distilled water in cell
Room temperature kept constant
4 - 15
Baseline (emission
wavelength 397 nm)
D
15 minutes
Drift:
D/A x 100  3%/15min