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CHROMASTER 5160
Hitachi CHROMASTER 5160 Manuals
Manuals and User Guides for Hitachi CHROMASTER 5160. We have
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Hitachi CHROMASTER 5160 manual available for free PDF download: Instruction Manual
Hitachi CHROMASTER 5160 Instruction Manual (1022 pages)
Brand:
Hitachi
| Category:
Water Pump
| Size: 14.36 MB
Table of Contents
Section 1
20
Preface
3
About this Manual
4
Important
5
Warranty on Product
5
Installation, Relocation and After-Sale Technical Service
7
Technical Seminars and Training Courses for Users
8
Estimated Life Time of the Instrument
8
Caution on Disposal of Instrument
9
Disposal of this Instrument and Its Parts
9
Precautions on CE Conformity Marking
9
Other Precautions
10
Information for Users on WEEE (Only for EU Countries)
10
Safety Summary
12
General Safety Precautions
13
WARNING Precautions in the Manual
14
CAUTION Precautions in the Manual
16
WARNING and CAUTION Labels on Instrument
17
NOTICE Precautions
18
Table of Contents
20
Outline
23
Isocratic System
23
Low-Pressure Gradient System
24
Functions
26
Name and Function of each Part on Pump
26
Name and Function of each Part on UI Pad (Option)
31
Specifications
35
Operation
38
Basic Operation
38
Power Supply
38
Parameter Setting
44
Operational State
46
ON/OFF Setting
47
Purging Operation
47
Manual Operation
48
Programming Operation
50
Washing after Operation
52
Exit Operation
52
UTLTY Setting
55
Zero Pressure Calibration
55
Pump Motor Control Setting
56
Purging Flow Rate Setting
57
Flow Rate Calibration Setting
58
Pressure Calibration Setting
59
Pressure Unit Setting
60
Column Flush Setting
61
Event Code Setting
64
Link Program Function
66
Error out Signal Setting
75
Plunger Wash Pump Setting
75
Leak Sensor Setting
76
Glp
77
Pump Seal Logbook
77
Plunger Wash Pump Logbook
78
Auto Purge Valve Logbook
79
Dynamic Mixer Logbook
80
Low Gradient Unit Logbook
81
Pressure Monitor
83
Key Lock
84
Analysis Operation
85
Analyzing Operation
87
Operation Procedure
87
Setup
98
Pump Mode Setting
98
LCD Contrast Setting
100
Before Performing Analysis
101
Selection of Mobile Phase and Cautions on Handling
103
Characteristics of Organic Solvents
106
Cautions on Static Electricity
111
Preparation
112
Degassing and Removing Dust from Mobile Phase
112
Degassing Mobile Phase
112
Removing Dust from Mobile Phase
115
Sample Pretreatment
116
Maintenance & Inspection
117
Performance and Specifications Check
117
Performance Check Preparation
117
Preparing the System for Operation
118
Flow Rate Precision Check
121
Periodic Maintenance
123
Liquid Leak Sensor Check
123
Periodical Inspection
125
Section 2
209
General
211
Configuration of Liquid Chromatograph System
213
Outline of Chromaster Organizer
213
Isocratic System
214
Low-Pressure Gradient System
215
Functions
217
Name and Function of each Part on Chromaster Organizer
217
Specifications
219
Fuse Replacement
220
Replacement Parts
220
Replacement Procedure
220
Troubleshooting
222
Index
249
Section 3
266
Isocratic System
269
Overview
269
Explanation of Power Switch and Others on Front Panel
273
Functions
273
Part Names and Functions of UI Pad (Option)
274
Rear Panel
276
Principle of Operation
277
Specifications
279
Basic Operations
283
Operation
283
Turning on Power Supply
283
Parameter Input Method
287
Auto Zero
290
Setting of Measuring Wavelength
290
Shutdown
291
Washing after Use
291
Setting the Offset
292
Utility Setting
292
Setting the Time Constant
294
Setting the Use or Non-Use of Time Program
295
Setting the Polarity
296
Other Settings
297
GLP-Related Functions
303
Logbook of D2/W/Hg Lamp
304
Key Lock
308
Checking the Energy and Wavelength Accuracy of D2 Lamp
309
Checking the Energy of W Lamp
310
Checking the Wavelength Accuracy with Hg Lamp
311
Analyzing Operation
312
Spectrum Measurement
312
Setting of Recorder and Spectrum Output
318
Operation of Marker
320
Setting of Time Program
320
Example of Time Program Operation
322
Execution of Time Program
322
Stop of Time Program
322
Setups
326
Selection of Communication Channel
327
Setting of Thermostatic Flow Cell
328
Setting of LCD Contrast
329
Before Analysis
330
Precautions on Selection and Handling of Mobile Phase
331
Characteristics of Organic Solvents
335
Caution on Static Electricity
339
Degassing and Decontamination of Mobile Phase
340
Preparatory Operation
340
Degassing of Mobile Phase
341
Decontamination of Mobile Phase
344
Sample Treatment
345
Confirmation of Basic Actions
346
Maintenance and Checkup
346
Confirmation of Performance Specifications
347
Items of Performance Specification Check
347
Measurement of Baseline Noise
348
Measurement of Baseline Drift
350
Check of Liquid Leak Sensor
352
Periodic Checkup
352
Periodic Maintenance/Checkup
354
Section 4
436
Isocratic System
439
Outline
439
Low-Pressure Gradient System
440
Functions
441
Name and Function of each Part on FL Detector
441
Name and Function of each Part on UI Pad (Option)
443
Description of Fluorometry
446
Principle of Fluorometry
446
Advantages of Fluorometry
448
Notes on Fluorescence Analysis
451
Principle of Operation
456
Features of Light Source
457
Specifications
457
Basic Operation
461
Operation
461
Power Supply
461
Parameter Setting
464
Auto Zero Adjustment
466
Wavelength Setting
466
Flushing the Flow Cell after Use
467
Shutdown Procedure
467
Offset Setting
468
UTLTY Setting
468
Time Constant Setting
469
Use Time Program Setting
470
PMT Voltage Setting
471
Other Settings
472
GLP Functions
477
Xe Lamp Logbook Data Display
477
Xe Lamp Logbook Data Resetting
478
Hg Lamp Logbook Data Display
480
Hg Lamp Logbook Data Resetting
481
Key Lock Setting
483
Wavelength Accuracy Check by Using Hg Lamp
484
Xe Lamp Energy Check
485
Operating Instructions
487
Time Program Setting
487
Time Program Operation Examples
493
Time Program Starting
493
Time Program Stopping
493
Spectrum Measurement
497
Recorder and Spectrum Output Factors Setting
501
Marker Operation
505
Communication Channel (CH) Setting
506
Set up Setting
506
Thermo Cell Setting
507
Liquid Crystal Display Contrast Setting
509
Standard Xenon Lamp Lifetime Setting
510
Before Performing Analysis
512
Characteristics of Organic Solvents
513
Selection of Mobile Phase and Cautions on Handling
513
Cautions on Static Electricity
520
Degassing and Removing Dust from Mobile Phase
521
Degassing Mobile Phase
521
Preparation
521
Removing Dust from Mobile Phase
524
Sample Pretreatment
525
Maintenance and Troubleshooting
527
Operational Check
527
Performance Checks
527
Xe Lamp Energy Check
527
Sensitivity Check
528
Wavelength Accuracy Check by Using Hg Lamp
528
Wavelength Accuracy Check and Calibration
541
Leak Sensor Check
544
Drain Tray Check
546
Periodic Checks
547
Wavelength Accuracy Check Using Hg Lamp
547
Xe Lamp Energy Check
547
Lamp Logbook Check
548
Flow Cell Unit Checks
549
Check and Washing of Flow Cell
550
Disassembly and Cleaning of Flow Cell
552
Leakage from the Flow Cell
555
Cautions on Storage of the Flow Cell
556
Troubleshooting
557
Error Messages
559
Replacement Parts and Consumables
562
Spare Parts
562
Lamp Replacement
564
Xe Lamp Replacement
564
Hg Lamp Replacement
578
Fuse Replacement
581
Index
582
Appendix
586
Appendix 1. Installation
586
Unpacking
586
Installation Conditions
587
Installation Environment
590
Installation Place
590
Checking the Contents
591
Assembling
592
Removal of Transport Screws
592
Appendix 2. Description on Contact Signal Communication
602
Contact Signals Entering the E-Line Connector
602
Outline
602
Individual Contacts of each Unit
605
Appendix 3. Glossary
607
Flowpath Figures
610
Isocratic System
610
Low Pressure Gradient System
610
Section 5
631
About this Manual
743
Preface
743
Important
744
Warranty on Product
744
Installation, Relocation and After-Sale Technical Service
746
Estimated Life Time of the Instrument
747
Technical Seminars and Training Courses for Users
747
Other Precautions
749
Section 6
755
Functions
757
Specifications
758
Operation
760
Preface
773
About this Manual
774
Important
775
Warranty on Product
775
Installation, Relocation and After-Sale Technical Service
777
Estimated Life Time of the Instrument
778
Technical Seminars and Training Courses for Users
778
Caution on Disposal of Instrument
779
Disposal of this Instrument and Its Parts
779
Precautions on CE Conformity Marking
779
Information for Users on WEEE (Only for EU Countries)
780
Warning
783
Caution
786
Section 7
791
Isocratic System
794
Outline
794
Low-Pressure Gradient System
795
Functions
797
Name and Function of each Part on Autosampler
797
The Instrument Keypad
797
Name and Function of each Part on UI Pad (Option)
801
Application
803
Configuration of Flow Path
803
Operating Principle and Functional Description
803
Sample Injection Method
805
Sample Injection Procedure
809
Main Unit
840
Specifications
840
Thermostat (Option)
843
Basic Operation
845
Operation
845
Power Supply
845
Parameter Setting
848
Basics of Operation
850
Measuring Operation
850
Analyzing Operation
856
Shutdown Operation
902
Before Performing Analysis
903
Selection of Mobile Phase and Cautions on Handling
905
Degassing of Mobile Phase and Wash Fluid
908
Removing Dust from Mobile Phase and Wash Fluid
909
Characteristics of Organic Solvents
910
Cautions on Static Electricity
914
Cautions on Measurement (Handling)
915
Preparatory Operations
915
Sample Pretreatment
916
Preparatory Operations of Autosampler Proper
917
Precautions on Handling
919
Checking the Performance and Specifications
922
Maintenance
922
Preparation of Items
922
Preparation of Operation
923
Reproducibility Check
926
Cleaning
929
Liquid Leak Check
929
Liquid Leak Sensor
929
Periodic Maintenance
929
Washing the Flow Path
929
Waste Solution Tube
929
Drain Tray
932
Cleaning and Positioning the Wash Port
933
Positioning the Mechanism
933
Troubleshooting
937
Troubleshooting Table
937
Autosampler Main Unit Errors
940
Error Messages
940
Thermostat (Option) Errors
943
Consumables
944
Consumables and Spare Parts
944
Parts Replacement
944
Spare Parts
945
Fastening of SUS Pipe
946
Replacement of Injection Port Seal
947
Needle Replacement
949
Syringe Replacement
951
Discharge of Air Bubbles from Syringe
955
Replacing the Injection Valve Seal
959
Replacing the Syringe Valve Seal
961
Purging the Wash Pump
963
Discharge of Air Bubbles from Wash Pump
964
How to Wash Solvent Filter
966
How to Replace Fuse (in the Optional Thermostat)
968
Accessories
969
Thermostat
969
For Thermostat
970
Sample Racks
970
Standard
970
Vials
971
Degassing Unit
982
Optional Syringes
982
Index
983
Appendix
987
Appendix 1. Installation
987
Appendix 1.1 Unpacking
987
Appendix 1.2 Place for Installation
988
Appendix 1.3 Items to be Prepared by Customer
990
Appendix 1.3.1 Power Supply Arrangement
990
Appendix
997
Appendix 1.3.2 Installation Space
997
Checking the Contents
997
Appendix
998
Appendix 1.5.1 Removal of Transport Screws
998
Assembling
998
Appendix
1000
Appendix 1.5.2 Attachment of Waste Solution Tube
1000
Appendix
1001
System Power Supply
1001
Appendix
1004
System Cabling
1004
Appendix
1006
System Tubing
1006
Appendix
1014
Appendix 1.5.7 Fastening between Component Units
1014
GUI Controller
1014
Re-Transporting Method
1015
Appendix 2. Explanation of Contact Signals
1017
Appendix 2.1 Introduction
1017
Appendix 2.2 Contact Signals Entering E-Line Connectors
1017
Appendix 2.3 Individual Contacts of each Unit
1019
Appendix 3. Terminology
1021
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