Spectrum Measurement - Hitachi CHROMASTER 5160 Instruction Manual

3.4.5 Spectrum measurement

Set the wavelength scan range (maximum and minimum values)
for excitation (Ex) and emission (Em), then measure and record
the spectral data for each.
* Carry out recording of spectral data with sample contained in
the cell (sample not flowing, but stationary in cell).
The spectral bandwidth is 15 nm on both excitation and
NOTE:
emission sides (bottom width of peak is about 30 nm).
A component with a difference between excitation and
emission wavelengths of less than 30 nm cannot be
accurately measured. Excitation and emission
wavelengths should be kept more than 30 nm apart for
measurement.
The maximum wavelength scan range (from end to
NOTE:
start wavelength) for both excitation and emission is
400 nm.
For spectrum acquisition in a range greater than
400 nm, the range should be divided into two for
measurement.
Saved spectral data will not be retained when the
NOTE:
power is turned OFF.
(1) Measurement and Recording of Spectrum for Excitation
(Ex) Scan Range:
(a) Press the SPECT key.
(b) The scan mode setting screen will appear.
<Scan mode setting screen>
SPECTRUM MODE (FL (Ex)=1, FL (Em)=2)
1
Press numeric key 1 and then the ENT to select FL
(Ex).
Now proceed to the scan range setting screen for
setting a scan range, and enter a start wavelength (a
cursor will appear at WL1).
(c) Enter a start wavelength (minimum value) at WL1 of
the scan range.
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