40
35
30
25
20
15
10
5
0
100
120
Fig. 2-8 All Volume Injection Method
Full Loop Injection Method
When applying Full loop injection method, the sample
introduction volume more than quintuple loop capacity
(injection volume) is required (amount of sample
necessity).
Select sample introduction volume within the area
indicated in the following figure.
The sample introduction volume is selected within the
shadow area of the following figure to fill a loop with the
density of the 99% of the sample. (For example, more
than 25 µL sample is required when injecting 5 µL
sample (5 µL loop capacity). This means that quintuple
sample volume is required.)
20
15
10
5
25
Amount of sample necessity introduction [µl]
Fig. 2-9 Full Loop Injection Method
2 - 11
140
160
Loop Capacity [µl]
50
75
180
200
100