Hitachi CHROMASTER 5160 Instruction Manual page 672

3.6 Before Performing Analysis
3.6.1 Selection of Mobile Phase and Cautions on Handling
Inflammation or Injury due to Toxic, Corrosive or
Stimulative Solvent
When using a toxic, corrosive or stimulative solvent, be
careful not to incur a physical inflammation or injury.
For details of the properties of each solvent and how to
handle it, refer to the relevant Material Safety Data Sheets
(MSDS). Be sure to handle each solvent properly.
(a) Wear proper protective clothes (e.g., safety goggles)
so that a solvent will not come into direct with the skin.
(b) Ventilate the laboratory room adequately to prevent
accidental inhalation of harmful solvent vapor.
(1) Be careful about restriction on use due to characteristics
and temperature of mobile phase to be used. (See Table
3-3 Characteristics of Organic Solvents)
(2) When performing high sensitivity analysis with a UV
detector, use solvents whose UV absorption characteristics
are especially small. Use solvents for liquid
chromatograph commercially available.
Absorption characteristics of special grade reagents such
as methanol, ethanol and acetonitrile generally and widely
used are greatly different in the short wavelength range,
depending on the manufacturing lot and the manufacturer.
Be careful especially when performing high sensitivity
analysis.
(3) Degas the mobile phase before use without fail.
(a) It is recommended to use a degassing unit because
organic solvents have a specially high content of air,
and the baselines may be fluctuated by bubbles.
Malfunction of check valve and loss of column
efficiency may also be caused. Degassing unit is
especially required for mixing solvents for gradient use.
Degas the solvents before use seeing 3.7.2 Degassing
Mobile Phase.
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WARNING