Hitachi CHROMASTER 5160 Instruction Manual page 953

(7) Before mounting a new syringe, put distilled water (or wash
fluid) into it. Avoid air bubbles in the plunger.
Plunger
Syringe
Beaker
Fig. 5-8 Filling with Distilled Water
(8) Mount the syringe (secure the top screw after engaging the
plunger groove with the hook). Be sure to engage the
plunger groove with the hook positively.
(9) Press the IDLE key on the UI pad. (Air bubbles entered at
the time of syringe mounting will be purged through port 4
(PUMP PLUNGER).
(10) Secure the plunger setscrew with your hand to fasten the
plunger groove with the hook
(11) Press the SPURGE key to replace fluid with wash fluid of
the syringe.
(Refer to 3.2.2 Analyzing Operation (5) Functions (g)
SPURGE for details.)
Or refer to 5.6 Discharge of Air Bubbles from Syringe to
avoid air bubbles.
(12) Press the IDLE key after you have completed eliminating
air bubbles.
(13) Attach the sealing screw on the port4 of the syringe valve.
NOTES: 1. While the inside of the syringe is dry, avoid moving
the plunger unless absolutely necessary.
Otherwise, the plunger tip will wear out abnormally.
5 - 10
Fill the syringe with distilled
water by pulling the plunger.
Distilled water
(or wash fluid)