Hitachi CHROMASTER 5160 Instruction Manual page 811

Syringe valve
4
vd
Plunger
wash
Syringe
Wash
fluid1
(2) Aspiration of the wash fluid1
The syringe aspirates the dead volume (:vd, inside parameter)
of fluid1. Switch the syringe valve to the wash port side
(port1) after aspiration of the wash fluid1.
When [wash before sample aspiration] is set, refer to the
following operation.
Washing of the needle outer wall and the injection port flow
path.
Deliver the wash fluid1 with the wash pump, move the needle
into the wash port, and wash the needle outer wall.
Wash the injection port flow path with the wash fluid1 after the
needle has moved into the wash port (the same as the
procedure (9)).
And when [fluid2 wash] is set, wash the injection port flow paths
using the wash fluid2 (the same as the procedure (10)).
Buffer tube
Injection valve
3
2
1
5
Wash
pump
P
3-way solenoid valve
Degassing
unit
v d :Dead volume (the instrument inside parameter)
v i :Injection volume (the sample net volume to the flow path)
v l :Lead volume (forward part, cut volume)
v r :Rear volume (rearward part, cut volume)
v a :Air volume (the air volume which sample is sandwiched.)
Wash
fluid2
Injection port
5
4
Loop
6
Waste
1 2
solution
To Column
From Pump
2 - 15
Needle
3
Waste
solution
Wash port
Rack
Sample