Periodic Checks; Xe Lamp Energy Check; Wavelength Accuracy Check Using Hg Lamp - Hitachi CHROMASTER 5160 Instruction Manual

4.3 Periodic Checks

No. Check Item
1 Drain tray
2 Lighting of lamp
3 Liquid leakage
4 Fluorescence
intensity check
5 Lifetime of lamp
6 Leak sensor
7 Wavelength
accuracy

4.3.1 Xe lamp energy check

4.3.2 Wavelength accuracy check using Hg lamp

Frequency of
Check
Daily Check the drain tray, and wash it when
needed.
Daily Check that "Xe" is indicated on LEDs.
Daily Visually check that liquid is not leaking
from the mobile phase bottle, tubing
and waste bottle.
Daily Press A/Z key, and check if FL
indication is zeroed.
Monthly 1) Check lamp for service life by
2) Check if baseline drifts considerably
Monthly 1) Drip water onto the leak sensor and
2) After confirmation, wipe off water.
Yearly Check wavelength by using bright line
of Hg lamp at 254 nm.
NOTE: First adjust the xenon lamp position (see 6.2.1 (3)), then
make an energy check of the xenon lamp and a
wavelength accuracy check using the Hg lamp.
Correct measurement results will not be obtained
unless the lamp position is adjusted.
Check the energy level of the Xe lamp at a wavelength of
254 nm (see section 3.3.7).
When the energy level of the Xe lamp has decreased
NOTE:
by 50% or more in comparison with the level at initial
use, the useful life of the lamp is considered to have
ended. The lamp life can be judged by the energy
level, or by baseline fluctuation (fluctuation of data
and/or noise).
Check the wavelength accuracy by means of the bright line of
the Hg lamp at 254 nm (see section 3.3.6).
"GOOD" will be indicated on the display when wavelength error
is within ±3 nm; "FAIL" will be indicated if the error exceeds
±3 nm.
4 - 21
Check Procedure
energy check, or by cumulative
lamp turn-on time.
(or if baseline fluctuates or noise
becomes high).
confirm the occurrence of an error.
Remarks
See 3.3
See 4.2.5
See 4.2.4