Sample Pretreatment - Hitachi CHROMASTER 5160 Instruction Manual

3.7.4 Sample Pretreatment

Filtered clean sample
Fig. 3-11 Example of Filtering Samples
(1) Filter samples without fail if they may contain fine dust.
Dust can clog column and deteriorate it.
(2) Remove foreign matter from samples without fail.
Foreign matter can lower the reliability of data, e.g.
generating abnormal peaks etc.
(a) Example of Filtering Samples
Dust in sample can be removed easily as follows.
Take sample into a syringe, attach cartridge type
0.45 m membrane filter, and perform filtering.
See the figure at left.
NOTE: There are various ways of filtering sample.
(b) Example of Removing Foreign Matter from Sample
For example, when analysis is performed with a
reversed phase column (e.g. ODS column), foreign
matter can be removed as follows. Take sample into
a syringe, attach a normal phase cartridge, and
perform filtering.
NOTE: There are various ways of removing foreign
Filtering by cartridge
type 0.45 m
membrane filter.
Filter the sample in the way suitable for the
sample amount.
See the figure at left.
matter from sample. Remove in the way
suitable for the purpose of analysis.
Fig. 3-12 Example of Removing Foreign
Matter from Sample
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Cartridge of silica gel
Clean sample after
removing foreign matter.