Operation Procedure; Analyzing Operation - Hitachi CHROMASTER 5160 Instruction Manual

3.4.1 Operation Procedure

3.4.2 Analyzing Operation

When the settings are all finished, carry out operation in the
following procedure.
(1) Make sure the pump, column, detector and data processor
are properly connected.
(2) Set the mobile phase and waste solution bottle in place.
(3) Start the pump and wait until the flow path is filled with the
mobile phase.
(4) Warm up the pump, column and detector and wait until the
baseline stabilizes.
(5) Inject a sample.
(6) When analysis is finished, turn the power off and dispose
of the waste solution.
NOTE: Before connecting a new column, make sure the flow
path is filled with mobile phase so that air won't enter
the column.
NOTE: The whirring sound of the pump during operation
varies depending on such operating conditions as flow
rate and pressure. Although the whirring sound may
become larger anywhere in the high-pressure range, it
is not a symptom of abnormality.
(1) SET PROGRAM allows the generation of a maximum of 9
programs.
You can execute any of the programs selected or
generated via the SET PROGRAM. For a gradient
analysis, implement the following procedure.
(a) Select [INITIAL], and select or generate a program for
mobile phase.
(b) Turn on each pump.
(c) Start the program.
(2) Contents of SET PROGRAM
For the SET PROGRAM, enter the upper limit pressure
level, lower limit pressure level, mixing ratio, flow rate, and
event.
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