Hitachi CHROMASTER 5160 Instruction Manual page 673

(b) When performing analysis with a UV detector in the
short wavelength range or highly sensitive analysis
with an RI detector, degassing the mobile phase
before use is not enough, and the baselines may be
fluctuated by the bubbles. In such a case, use a
degassing unit which can degas mobile phase on-line.
(4) Mobile Phase
When using mobile phase for liquid chromatograph, since
the connections of pipings of pump and separation column
use stainless steel (SUS316), solvents or reagents that
may corrode stainless steel may be restricted.
Especially, solvents or solutions which contain halogen
ions such as HCl, KCl, NaCl, NH
Reagents which may be restricted are shown in Table 3-2,
use the table as a rough standard.
NOTE: When unsuitable reagents absolutely necessary for
ingredient separation or reaction of a constituent have
been used, or when mobile phase which deposits salt
has been used, wash all the flow paths with distilled
water after completing analysis without fail. If not, the
plunger may wear down. Also, for preventing
precipitation of salt on the plunger and its seal, inject
water into the plunger washing mechanism at the rear
of the pump head. Water should be injected with the
furnished cylinder connected to the connector of the
washing mechanism. When the optional plunger
washing pump is connected, wash the plunger by
driving this pump.
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Cl are typical reagents.
4
3.6.1