Hitachi CHROMASTER 5160 Instruction Manual page 683

3.7.3 Removing Dust from Mobile Phase
(h) Turn off the power of ultrasonic cleaner.
(i) Take mobile phase container out of ultrasonic cleaner,
wipe the water from the container, and set it to liquid
chromatograph.
NOTE: Bubble generation depends on constitution of mobile
phase. When mobile phase is a mixture with organic
solvent (e.g. methanol 20% + water 80%), fix the
degassing time because constitution ratio of mobile
phase may be varied by the degassing time (boiling
point of mobile phase would vary with a reduced
pressure).
There are various ways to remove dust from mobile phase.
Remove dust in the way suitable for the purpose of use.
(1) Example of Filtering of Mobile Phase by Using Membrane
Filter
Removing dust and degassing can be performed
simultaneously by filtration with a 0.45 μm membrane filter.
Membrane filter
NOTE: Remove dust from mobile phase by using a
0.45 μm membrane filter. (Do not use filter paper
because it generates dust.)
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3.7.3