Description Of Fluorometry; Principle Of Fluorometry - Hitachi CHROMASTER 5160 Instruction Manual

2.3 Description of Fluorometry

2.3.1 Principle of fluorometry

Excitation
Excited state V =
Excited triplet
state
Excitation light
Ground state V =
Stable condition
(Ground state)
3
2
1
0
3
2
1
0
Absorption
Fig. 2-4 Typical Organic Molecular Energy Level Diagram
Figure 3-4 shows the energy level transitions of an organic
molecule for the various processes involved in absorption,
fluorescence and phosphorescence.
Fluorescence or
Phosphorescence
Radiationless
transition
Light
Fluorescence
2 - 6
Unstable condition
(Excited state)
Radiationless
transition
Light
Phosphorescence