Hitachi CHROMASTER 5160 Instruction Manual page 927

(3) Press the WASH key of the autosampler, and select
NEEDLE side to fill the needle and the injection port with
wash fluid1. (When two wash fluids are used, replace the
wash fluid2 side with fresh one). Fill the buffer tube with
deaerated wash fluid while flushing the needle and the
injection tube.
(4) Place a vial containing at least 1 mL of standard sample in
rack position No. 1 and set the rack in the autosampler.
(5) Press the START key of autosampler to start analysis.
The analysis is carried out 6 times. The operating
conditions during analysis are indicated on the display.
Ensure beforehand that the room temperature will not
change suddenly during analysis.
(6) Upon completion of analysis, the operation of the system
stops with the initial screen displayed. Instead of the initial
screen, the ALL END screen will appear if ALL END is set.
In this case, the initial screen appears when you press the
IDLE key.
(7) Data Check
Determine the Relative Standard Deviation (RSD) of peak
area by the following calculations.
Mean value
Standard deviation
Relative Standard Deviation
xi: Area value due to each injection,
n: Number of injections
Make sure the CV value is 0.2% or less (injection volume is
10 µL or less). However, this value represents the
reproducibility of not the autosampler alone but the entire
system. Therefore, the value varies with each stability of
the pump and detector, accuracy of the data processing
unit and its processing parameters.
4 - 6
n
∑
xi
=
=
i 1
x
n
n
∑ −
2
( xi ) x
=
=
i 1
SD
−
n 1
SD
= ×
RSD 100
x
(%)