Preparatory Operations Of Autosampler Proper - Hitachi CHROMASTER 5160 Instruction Manual

3.5.3 Preparatory Operations of Autosampler Proper

(b) Example of Removing Foreign Matter from Sample
For example, when analysis is performed with a
reversed phase column (e.g. ODS column), foreign
matter can be removed as follows. Take sample into
a syringe, attach a normal phase cartridge, and
perform filtering.
NOTE: There are various ways of removing foreign
matter from sample. Remove in the way
suitable for the purpose of analysis.
(1) Check that the autosampler is correctly connected to the
pump and detector before turning on the power.
(2) Check that the drain tube is correctly connected to the
waste (solution) bottle. The waste bottle must be
positioned at a level lower than the autosampler.
(3) Prepare other components to be connected to the
autosampler, referring to the respective instruction manual.
(4) The drain tube of the detector must be placed in a waste
bottle that is dedicated for the detector.
(5) Connect the mobile phase bottle to the pump and connect
the detergent bottle to the autosampler.
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See the figure at left.
Cartridge of silica gel
Clean sample after
removing foreign matter.