Hitachi CHROMASTER 5160 Instruction Manual page 538

(ii) Check the position and useful life of the xenon
lamp.
(b) When peak separation is not good
(i) Check the distilled water.
* Distilled water should be used soon after its
purification. If left for a long time in a container
made of resin or the like, fluorescent substance
may mix in with the distilled water.
(ii) Check for bubbles
If water is directly injected into an empty flow cell,
small bubbles may remain in the cell. To
prevent this, first inject ethanol or the like and
then water.
(iii) Change the method of spectrum measurement
(via time program).
Measure the Raman spectrum of water using a
time program. But first check the fluctuation of
FL value via wavelength shift.
Set the excitation(Ex) wavelength at 350 nm and
emission(Em) wavelength at 450 nm using
WL
the key.
Then zero the FL value with the
Next, with the
wavelength near 397 nm where the FL value is
maximum, and the Em wavelength near 380 nm
where the FL value is minimum.
The minimum FL value should be less than half
the maximum FL value.
4 - 12
A/Z
WL
key, check the Em
key.