Hitachi CHROMASTER 5160 Instruction Manual page 522

Mobile phase container
(a) Set mobile phase container well stirred into ultrasonic
cleaner.
* When using a mixed mobile phase, stir it completely
by using a stirrer, or else low repeatability or
baseline drift may be caused.
NOTE: Degas mobile phase of organic solvent by using
ultrasonic cleaner with water.
(b) Turn on the power of ultrasonic cleaner.
(c) When degassing, small bubbles are generated first,
then gradually larger bubbles are generated.
Continue degassing until bubbles are not generated
anymore.
It takes 5 to 10 minutes to finish.
(d) When bubble generation finishes, turn off the power of
ultrasonic cleaner.
(e) Take out mobile phase container from ultrasonic
cleaner, wipe the water from the container, and set the
container to liquid chromatograph.
(2) Example of Degassing by Reducing Pressure (mobile
phase is aqueous solution)
Example: Ethanol 20% + water 80%, 0.2% citric acid
aqueous solution
3 - 62
Remove cap.
Ultrasonic cleaner