Hitachi CHROMASTER 5160 Instruction Manual page 831

Full Loop Injection Method Procedure
The full loop injection method, the injection volume, the west
volume, and the air volume are restricted by the following relational
expression. A stroke of the syringe capacity can't be used all.
<175 µL of Standard syringe >
vi + vw + va ≦ 165 µL
vi :Injection volume (The sample net volume to the flow path)
vw :Waste volume (The disposal sample volume of the lead and rear
part of the loop)
va :Air volume (The air volume of the lead and rear part of a sample)
One-half of the waste volume (vw/2) is calculated and delivered
automatically to the lead and the rear part of the loop each.
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