Hitachi CHROMASTER 5160 Instruction Manual page 514

(b) When performing analysis with a UV detector in the
short wavelength range or highly sensitive analysis
with an RI detector, degassing the mobile phase before
use is not enough, and the baselines may be fluctuated
by the bubbles. In such a case, use degassing unit
which can degas mobile phase on-line.
(4) Mobile Phase
When using mobile phase for liquid chromatograph, since
the connections of pipings of pump and separation column
use stainless steel (SUS316), solvents or reagents that may
corrode stainless steel may be restricted.
Especially, solvents or solutions which contain halogen ions
such as HCl, KCl, NaCl, NH
Reagents which may be restricted are shown in Table 4-1;
use the table as a rough standard.
NOTE: When unsuitable reagents absolutely necessary for
ingredient separation or reaction of a constituent have
been used, or when mobile phase which deposits salt
has been used, wash all the flow paths with distilled
water after completing analysis without fail. If not, the
plunger may wear down.
Pour water into the plunger wash mechanism in the rear
part of the pump head and wash to prevent the plunger
and the plunger seal from salt deposition.
Connect an accessory cylinder to the connector on the
wash mechanism and pour water.
Run the plunger wash pump when the optional plunger
wash pump is connected.
3 - 54
Cl are typical reagents.
4